▎ 摘　　要

Modern society envisions hydrogen (H-2) fuel to drive the transportation, industrial, and domestic sectors. Here, we explore use of graphene oxide nanoparticles (GO NPs) for greatly enhancing bio-H-2 production by a consortium based on Thermoanaerobacterium thermosaccharolyticum spp. Thermophilic batch bioreactors were set up at 60 degrees C and initial pH of 8.5 to assess the effects of GO NPs supplements on biohydrogen production. Under optimal GO NPs loading of 10 mg/L, the supplemented system yielded similar to 300% higher H-2 yield (6.78 mol H-2/mol sucrose) than control. Such an optimized system offered 73% H-2 purity and 85% conversion efficiency by promoted the desirable acetate fermentation pathway. Miseq Illumina sequencing tests revealed that the optimal levels of GO NPs did not alter the microbial composition of consortium.