▎ 摘　　要

Ceramide exerts crucial effect on inducing tumor cell apoptosis, while its insolublility limits the application in treating tumors. In this study, we used NGO-PEG-PEI (NPP) and C6-NPP/Cer (NPP/C) as method to explore NPP/C's effect and its anti-tumor ability on breast cancer. Confocal microscopy was used to detect the transfection efficiency in tumor cells. Breast cancer cells were treated with C6-NGO-PEG-PEI solution (control group) or NPP/C followed by analysis of cell proliferation and apoptosis by flow cytometry. C6-ceramide solution (control group) or NPP/C was administrated into nude mice with tumor followed by measuring tumor volume and size as well as cell proliferation and apoptosis. NGO-PEG-PEI could significantly enhance cell intake and inhibit cell proliferation and promote apoptosis. In vivo transplantation tumor model experiments showed that NPP/C could decrease tumor growth, slow down the multiplication rate and accelerate apoptosis. In conclusion, Ceramide-graphene oxide can inhibit tumor growth by inhibiting tumor cell growth and promoting cell apoptosis.