▎ 摘　　要

Acetylcholine and dopamine are the most important neurotransmitters in the nervous system. Lack of the balance in their activity can cause serious mental disorders. So, it is important to detect the trace levels of these compounds by rapid and simple methods. Herein, nanosized needle-shaped WO3 as a two-dimensional metal oxide was synthesized and hybridized with graphene oxide and applied for modification of a graphite pencil electrode (WO3/GO-pencil). The synthetic material was characterized with X-ray diffraction (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images and applied for fabrication of an electrochemical electrode. The as-prepared sensor was used successfully for simultaneous detection of acetylcholine and dopamine in real body fluids via electrocatalytic oxidation of the compounds. Dopamine and acetylcholine were detected concurrently by the stable and repeatable WO3/GO-pencil in the very low levels (2.5 and 3.7 nM, respectively). Also, the fabricated sensor showed a good stability with a significant RSD% about 2.67 % for reproducibility. The sensor performance was Eventually validated by the high performance liquid chromatography (HPLC) as a reliable method.