• 文献标题:   Increased lipid peroxidation by graphene quantum dots induces ferroptosis in macrophages
  • 文献类型:   Article
  • 作  者:   SHAO Y, WANG LT, CHEN JJ, HUNAG YY, HUANG YW, WANG XY, ZHOU DX, ZHANG JQ, WU W, ZHANG QY, LI F, XIA XF, HUANG Y
  • 作者关键词:   graphene quantum dot, proteomic, lipdomic, glutathione peroxidase 4, ferroptosi
  • 出版物名称:   NANOIMPACT
  • ISSN:   2452-0748
  • 通讯作者地址:  
  • 被引频次:   4
  • DOI:   10.1016/j.impact.2021.100334 EA JUN 2021
  • 出版年:   2021

▎ 摘  要

Graphene quantum dots (GQDs) are an excellent tool for theranostics, and are widely used in nanomedical applications. The biosafety of GQDs has received abundant attention, but their latent toxicological mechanisms remain inadequately understood. To investigate the cellular and molecular mechanisms underlying graphene-mediated changes, quantitative proteomics and untargeted lipidomics were integrated. We discovered that glutathione peroxidase 4 as a key regulator of ferroptosis, was down-regulated at the protein level by GQDs. Lipidomics profiling with features of ferroptosis was identified in GQDs-treated RAW264.7 macrophages. Furthermore, GQDs exposure was associated with reduced levels of GSH and increased lipid peroxidation. Overexpression of GPX4 in RAW264.7 cells and pre-treatment of a ferroptosis inhibitor Ferrostatin-1 (Fer-1) not only suppressed cell death, but also alleviated lipid peroxidation. Taken together, our results indicated that GQDs exposure induced ferroptosis in RAW264.7 macrophages, and provided essential data for biosafety evaluations of GQDs.