▎ 摘　　要

In this work, we presented a novel biosensor for rapid detection of paracetamol and ascorbic acid. The novel biosensor was based on the fluorescent "turn off-on" of polypyrrole/graphene quantum dots (PPy/GQDs) composites. The composites exhibit strong fluorescence emission, which is dramatically enhanced as high as three times than that of pure GQDs. It is found that the fluorescence intensity of PPy/GQDs can be efficiently quenched by N-acetyl-p-benzoquinone (4-AOBQ), the oxidation product of paracetamol (PAR). And a turn-on fluorescence signal was observed when 4-AOBQ is reduced by ascorbic acid (AA). The quenched and recovered fluorescence intensity of PPy-GQDs was proportional to the concentration of PAR (0.067-233 mu g/L) and AA (3.33-997.5 mu g/L) respectively. The limit of detection is 0.022 mu g/L for PAR and 1.05 mu g/L for AA. The present method was applied to the determination of PAR and AA in human serum samples with satisfactory results.