▎ 摘　　要

The antibacterial activity against Staphylococcus aureus (S. aureus) in diabetic foot wound treatment is an appealing area for budding researchers. In this case, drug-loaded chitosan (CS)/polyvinyl alcohol (PVA)/gra-phene oxide (GO)-based composites can be used as an excellent option for antibacterial activity in diabetic foot wound treatment. Therefore, the present study aims to design a pirfenidone-loaded CS/PVA/GO nanocomposite (PFD-CS/PVA/GO) based scaffold via solvent casting method for improved antibacterial activity. In brief, CS with PVA forms the polyelectrolyte complex due to hydrogen bonding between amine functionality (CS) and a hydroxyl group (PVA). The GO nanosheet addition into CS/PVA resulted in covalent bonding between the amine functionality (CS) and the carboxylic functionality (GO) whereas PFD was fixed in CS/PVA/GO via 7C -7C stacking. In this study, optimized PFD-CS/PVA/GO (6% w/w) scaffold percent entrapment efficiency, tensile strength, moisture content, % drug release, % swelling degree, % elongation at break, and water retention capacity were found to be 77.60%, 70.35 g/cm2, 16.39%, 50.60% (7 days), 236%, 45%, and 543.47%, respectively. Release kinetics assured that the Higuchi matrix was the best-fit model (R2 = 0.99). Interestingly, the GO avoids burst drug release at the beginning followed by extending the release whereas CS into PFD-CS/PVA/GO provides a good adhesive ability. Finally, antibacterial activity against S. aureus of PFD-CS/PVA/GO (6% w/w) shows a high (12.06 mm) zone of inhibition over a separate component of the scaffold. Concisely, optimized PFD-CS/PVA/GO (6% w/w) scaffolds provide improved antibacterial potential owing to their combined benefits of CS, and GO. In the future, anticipated PFD-CS/PVA/GO scaffolds will open a new door for antibacterial potential in diabetic foot wound healing.