▎ 摘　　要

Preventing fluorescence quenching caused by direct contact between fluorophores and metal particles is a pri-mary research direction of surface-enhanced fluorescence (SEF). In this paper, a silicon wafer/silver nano-particles/annealed graphene oxide (Si/AgNPs/aGO) composite structure substrate was designed for SEF. Annealed graphene oxide (aGO) nano-sheets acted as barrier between silver nanoparticles and fluorophores, reducing their direct contact to improve fluorescence performance. The gaps between silver nanoparticles (AgNPs) produced strong electromagnetic field, which was simulated by three-dimensional finite-difference time-domain (3D-FDTD). After adding the aGO nano-sheets spacer between the fluorescent molecules and silver nanoparticles, the fluorescence performance was further improved, which indirectly indicated the spacer effect of aGO. This strategy can prevent the fluorescence quenching effect caused by the direct contact between fluorescent molecules and metal particles. It is extremely helpful to improve the fluorescence enhancement effect.