▎ 摘　　要

Graphene oxide (GO) is acquiring a great interest in biomedicine, biotechnology and biochemistry due to its unique properties. However, GO layers are bound by van der Waals forces, which results in aggregation. An efficient dispersion of the aggregated nanostructures is crucial from an application viewpoint, hence eco-friendly procedures are pursued. In this work, the potential of tannic acid (TA) as a GO dispersant in water has been investigated for the first time. Transmission electronic microscopy (TEM) was used to visualize the degree of GO exfoliation in the dispersions. To further assess TA dispersant capability, a fluorescent biomolecule, riboflavin, has been selected. GO and TA cause a quenching effect on riboflavin fluorescence, which depends on the GO and TA concentration, the GO/TA weight ratio and the final centrifugation step that was found to be crucial. Multiple regression analysis has been used to determine the quenching constants for TA and GO simultaneously. The GO-riboflavin interaction weakens upon centrifugation. This step, traditionally used to remove the nanomaterial aggregates, should be avoided to obtain a high GO concentration in the dispersions. This study paves the way towards the use of environmentally friendly dispersant agents instead of conventional organic solvents or synthetic surfactants to attain high-quality dispersions of carbon nanomaterials in water. (C) 2022 The Author(s). Published by Elsevier B.V.