▎ 摘　　要

The entrapment of functionalized graphene, as filler, in polymeric conductive layers, such as polypyrrole (PPy), improves their engineering features. The objective was the association of graphene-PPy composite with nanosphere lithography generating hybrid polystyrene-graphene-PPy patterned platforms, seeking a further enhancement of their characteristics: an improved mechanical stability and electron transfer rate, combined with an increase of the active surface area. Additionally, the substrate was modified with N-hydroxysuccinimide activated carboxylic functional groups, for the controlled immobilization of Antiparacetamol antibody in order to elaborate an immunosensor for paracetamol detection. The behavior of the nanostructured hybrid platform was tested by imagistic, impedimetric and electrochemical quartz crystal microbalance experiments, which proved the successful patterning of the composite material and the biomolecule's immobilization. The sensor allowed the specific detection of paracetamol on a linear range of 0.1 to 25 mu M on the unstructured platform. The analytical signal was improved on the patterned material in comparison with the non-structured one, proving the superior nature of the structured surface.