▎ 摘　　要

Interrelated reaction networks steered by multiple types of enzymes are among the most intriguing enzyme-based cellular features. These reaction networks display advanced features such as adaptation, stimuli-responsiveness, and decision-making in accordance with environmental cues. However, artificial enzyme particles are still deficient in network-level capabilities, mostly because delicate enzymes are difficult to immobilize and assemble. In this study, we propose a general strategy to prepare enzyme-based particles that demonstrate network reaction capability. We assembled multiple types of proteins with a nanoscopic binder prepared from polyelectrolyte and graphene. After assembly, the enzymes all preserved their catalytic capabilities. By incorporating multiple types of enzymes, the particles additionally displayed network-reaction capabilities. We were able to use NIR irradiations to quasi-reversibly adjust the catalytic abilities of these enzyme-based particles. In addition, after a biomimetic mineralization process was used to wrap the protein complexes in MOF shell, the particles were more robust and catalytically active even after being immersed in acidic (pH 4) or basic (pH 10) solutions for 3 days. This study provides an insight into the study of network properties of functional enzyme particles experimentally and enriches scientific understanding of multifunctional or stimuli-responsive behaviors at the reaction network level. The building of artificial reaction networks possesses high potential in realizing intelligent microparticles that can perform complicated tasks.