• 文献标题:   Graphene oxide-based three-dimensional Au nanofilm with high-density and controllable hotspots: A powerful film-type SERS tag for immunochromatographic analysis of multiple mycotoxins in complex samples
  • 文献类型:   Article
  • 作  者:   ZHENG S, WANG CG, LI JX, WANG WQ, YU Q, WANG CW, WANG SQ
  • 作者关键词:   graphene oxidebased au nanofilm, builtin nanogap, mycotoxin, sersbased immunochromatographic assay, multiplex detection
  • 出版物名称:   CHEMICAL ENGINEERING JOURNAL
  • ISSN:   1385-8947 EI 1873-3212
  • 通讯作者地址:  
  • 被引频次:   9
  • DOI:   10.1016/j.cej.2022.137760 EA JUN 2022
  • 出版年:   2022

▎ 摘  要

Co-contamination of mycotoxins in food and environment is a common phenomenon, which easily causes cumulative and synergistic damaging effects to human and animal health, poses great health and economy burdens to the world. A convenient technology for detection of multiple and low-concentration mycotoxins in real samples is highly desired but remains a challenge. Here, we proposed a multiplexed surface-enhanced Raman scattering (SERS)-immunochromatographic assay (ICA) that can sensitively and simultaneously detect three mycotoxins in unprocessed complex samples, using a graphene oxide-based three-dimensional (3D) Au nanofilm (called GO@Au-Au) as the film-type SERS tag. A precise sub-1 nm PEI layer was constructed into the GO@Au-Au nanostructure as built-in nanogap, which can accommodate Raman reporter molecules and create stable hotspots between the inner GO@Au film and outer assembled AuNP satellites. Grafting the 30 nm AuNPs onto the 2D GO@Au nanofilm greatly improved the SERS activity and colorimetric signal of film-type tags. Compared with traditional spherical SERS tags, the film-type GO@Au-Au can provide greater reaction interface, excellent stability and dispersibility and multiple SERS hotspots over large area for ICA using. Given these advantages, the proposed SERS-ICA can achieve simultaneous quantitative detection of fumonisin B1, aflatoxin B1, and zearalenone, with low detection limit (0.529, 0.745, and 5.90 pg mL(-1)), short testing time (20 min), and high accuracy for real food/experimental samples. Our method shows great potential to fulfill practical requirements for detection of multiple mycotoxins.