▎ 摘　　要

According to studies, Hydrogen peroxide (H2O2) is a significant biomarker of physiological processes. Unnormal H2O2 levels in human body may result indiseases. Hence, there is an incrasing demand for monitoring the H2O2 concentraIP: 203.8.109 20 On: Thu, 22 Sep 2022 10:00:37 tions in biological specimen. Here, we construct a non-enzymatic H2O2 electrochemical biosensor based on persimmon tannin-reduced graphene oxide-platinum-palladium nanocomposite (PrG-Pt@Pd NPs) modified with screen-printed carDelivered by Ingenta bon electrode (SPE). Combined with suitable electrocatalytic mode for Pt@Pd NPs, high specific large specific volume and good electrical conductivity of RGO, well as the superior sorption capacity of PT for metal-based nano-ion, the PrGPt@Pd striped pleasing heterogeneous catalytic activity toward H2O2 reduction via the synergistic effect. In experimental conditions of optimal, this non-enzymatic electrochemical sensor exhibited excellent electrocatalytic performance for H2O2 with less negative potential (-0.5 V), fast response time (<3 s), it shows good linearity in the range of 5.0-100.0 mu M, in addition to this LOD of this sensor was 0.059 mu M as well as the excellent sensitivity of the sensor (13.696 mu A center dot mu M-1 center dot cm-2). Due to excellent specificity, lower detection limit, and good recovery (98.70-99.96%) in the spiked measurements of human serum samples, this non-enzymatic electrochemical biosensor paves the way for H2O2 detection at ultra-low concentrations in physiology and diagnosis.