▎ 摘　　要

A Pseudomonas fragi (P. fragi) and graphene-gold hybrid nanomaterial included a carbon felt electrode (graphene-Au/CFE) bioanode was developed and optimized. Then, this bioanode was combined with a platinum cathode and a single cell compartment microbial fuel cell (MFC) was formed. To the best of our knowledge, this is the first study that has utilized P. fragi as a biocatalyst. After the optimization of the working parameters of P. fragi/graphene-Au/CFE bioanode, like the effect of benzoquinone (BQ) on bioanode performance, BQ concentration optimization, P. fragi bacteria amount and graphene-Au hybrid nanomaterial amount optimization, the characteristics of the developed MFC were examined. As a result, 0.169 V OCP, 23.80 mu A cm(-2) polarization and 0.50 mu W cm(-2) power density values together with remarkable lifetime (28 days) were obtained.