▎ 摘　　要

A novel electrochemical sensor for the determination of tyramine (Tyr) based on a polymeric film of L-histidine coated on a screen-printed graphene electrode (poly(His)/SPGE) is presented for the first time. This developed sensor was constructed through a single modification step via an electropolymerization process. The poly(His) feature exhibited an outstanding electrocatalytic activity in the electrooxidation of Tyr. Under optimal conditions, the Tyr sensor revealed relatively broad two linear ranges of 0.5-20 mu M and 50-300 mu M with a limit of detection and limit of quantification of 0.065 mu M (3SD/slope) and 0.22 mu M (10SD/slope), respectively. Furthermore, the reliability of this proposed sensor was successfully investigated by detecting Tyr in both biological fluids and cheese samples, with satisfactory recovery ranges (82.58%-109.75%) and relative standard deviations (0.28%-8.03%). Therefore, the proposed sensor has distinct advantages, such as low cost, easy operation, disposable, high sensitivity, and good selectivity. On the merits of the aforementioned benefits, the newly developed electrochemical sensor could be a promising alternative approach for Tyr assessment in food quality and safety control, as well as a better understanding of clinical function associated with migraine triggers.