• 文献标题:   Chitosan and EDTA conjugated graphene oxide antinematodes in Eggplant: Toward improving plant immune response
  • 文献类型:   Article
  • 作  者:   ATTIA MS, ELSAYYAD GS, ABD ELKODOUS M, KHALIL WF, NOFEL MM, ABDELAZIZ AM, FARGHALI AA, ELBATAL AI, EL ROUBY WMA
  • 作者关键词:   antinematodal activity, gonanocomposite, meloidogyne incognita, chitosan
  • 出版物名称:   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
  • ISSN:   0141-8130 EI 1879-0003
  • 通讯作者地址:  
  • 被引频次:   21
  • DOI:   10.1016/j.ijbiomac.2021.03.005 EA MAR 2021
  • 出版年:   2021

▎ 摘  要

A new strategy regarding the fabrication of chitosan (CS) or ethylene diamine tetraacetic acid (EDTA) on graphene oxide (GO) was performed. The nematocidal potential against Meloidogyne incognita causing root-knot infection in eggplant was tested. The plant immune response was investigated through measuring the pho-tosynthetic pigments, phenols and proline contents, oxidative stress, and antioxidant enzymes activity. Results indicating that, the treatment by pure GO recorded the most mortality percentages of M. incognita 2nd juveniles followed by GO-CS then GO-EDTA. In vivo greenhouse experiments reveals that, the most potent treatment in re-ducing nematodes was GO-CS which recorded 85.42%, 75.3%, 55.5%, 87.81%, and 81.32% in numbers of 2nd juve-niles, galls, females, egg masses and the developmental stage, respectively. The highest chlorophyll a (104%), chlorophyll b (46%), total phenols (137.5%), and free proline (145.2%) were recorded in GO-CS. The highest malondialdehyde (MDA) value was achieved by GO-EDTA (7.22%), and hydrogen peroxide (H2O2) content by 47.51% after the treatment with pure GO. Treatment with GO-CS increased the activities of catalase (CAT) by 98.3%, peroxidase (POD) by 97.52%, polyphenol oxidase (PPO) by 113.8%, and superoxide dismutase (SOD) by 42.43%. The synthesized nanocomposites increases not only the nematocidal activity but also the plant sys-tematic immune response. (C) 2021 Elsevier B.V. All rights reserved.