▎ 摘　　要

Graphene oxide (GO) is an oxidized form of graphene that is relatively cheap and easy to produce. This has heralded its widespread use in a range of industries, with its likelihood of release into the environment increasing accordingly. In pure culture, GO has been shown to influence bacteria and fungi, but its effects on environmental microbial communities remain poorly characterized, despite the important ecosystem services that these organisms underpin. Here, we characterized the effects of GO and graphite, over time (7, 14 and 30 days) and at three concentrations (1 ng, 1 mu g and 1 mg kg dry soil(-1)), on soil bacterial and fungal diversity using 16S rRNA and ITS2 gene amplicon sequencing. Graphite was included as a reference material as it is widely distributed in the environment. Neither GO or graphite had significant effects on the alpha diversity of microbial communities. The composition of bacterial and fungal communities, however, was significantly influenced by both materials at all doses. With the exception of the lowest GO dose on day 14, these effects were apparent for all treatments over the course of the experiment. Nonetheless, the effects of GO and graphite were of similar magnitude, albeit with some differences in the taxa affected. (C) 2019 Elsevier B.V. All rights reserved.