▎ 摘　　要

A non-enzymatic amperometric sensor for hydrogen peroxide (H2O2) was developed based on the screen-printed electrode (SPCE) modified with reduced graphene oxide (RGO), polyneutral red (PNR) and gold nanoparticles (AuNP). Electrochemical behaviour of hydrogen peroxide on SPCE/RGO/PNR/AuNP was investigated. The prepared sensor showed a high electrocatalytic effect on the reduction of hydrogen peroxide. The non-enzymatic sensor response for H2O2 was investigated as a function of pH and working potential. Optimum values of these parameters in the H2O2 determination were found as 7.0 for pH and -0.45 V for working potential. Amperometry was used successfully for the determination of H2O2 in the linear response range from 21.16 to 2730 mu M with a detection limit of 6.35 mu M. The relative standard deviation (RSD) was calculated to be 1.70% (for n = 10). The operational stability studies have shown that the initial amperometric response of the sensor to H2O2 decreased by 60.82% on the 60th day. Storage life studies have shown that the sensitivity of the sensor decreased by 40.31% at the end of 12 weeks. The non-enzymatic sensor has been tested for H2O2 determination in human serum sample. The developed H2O2 sensor is promising to be used for H2O2 analysis in human serum samples as simple, practical and disposable device without requiring laborious sample pre-treatment producers.