▎ 摘　　要

Gene therapy has recently reached the front line in the treatments of different diseases. Although graphene oxide has been widely used as a gene vector, its clinical application is limited by the scarce inhomogeneity of the different synthetic methods. Recently, we have developed the preparation of a new class of graphene oxide, named oxo-G, obtained by a rigorous synthetic method with controlled size and surface chemistry. oxo-G has been successfully applied for the production of reduced graphene oxide with remarkable conductivity. Here, we applied oxo-G to gene delivery. After ad hoc functionalization, we found that oxo-G is able to efficiently complex siRNA and strongly induce gene silencing in vitro at five pg/cell doses, more than 20 times lower than the best results reported in the literature using graphenebased materials. The aim of this work is to inspire the readers to develop further oxo-G materials for biomedical applications. We hope that oxo-G can be considered as an alternative candidate in future gene silencing use in vivo.