• 文献标题:   Magnetic graphene oxide-based electrochemiluminescent aptasensor for thrombin
  • 文献类型:   Article
  • 作  者:   JIN GX, LU LJ, GAO XY, LI MJ, QIU B, LIN ZY, YANG HH, CHEN GN
  • 作者关键词:   magnetic graphene oxide, electrochemiluminescence ecl, thrombin, aptamer, iridium iii complex
  • 出版物名称:   ELECTROCHIMICA ACTA
  • ISSN:   0013-4686 EI 1873-3859
  • 通讯作者地址:   Fuzhou Univ
  • 被引频次:   22
  • DOI:   10.1016/j.electacta.2012.10.155
  • 出版年:   2013

▎ 摘  要

A new electrochemiluminescence (ECL) reagent, iridium(III) complex (Kill) complex), has been synthesized and applied to develop an ECL biosensor for thrombin. The Ir(III) complex has been conjugated to aptamers firstly and adsorbed on magneto-controlled magnetic graphene oxide. In the presence of the target (thrombin), the aptamers will release into solution and result in increased ECL intensity. The enhanced ECL intensity has a direct relationship with the logarithm of thrombin concentration in the range of 2.0 x 10(-9) to 5.0 x 10(-8) mol/L. The detection limit was 1.3 x 10(-9) mol/L (S/N = 3). The proposed method has good stability and reproducibility. (C) 2012 Elsevier Ltd. All rights reserved.