▎ 摘　　要

Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix. One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively. Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag. PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS). The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively. All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect). Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.