• 文献标题:   A highly sensitive competitive immunosensor based on branched polyethyleneimine functionalized reduced graphene oxide and gold nanoparticles modified electrode for detection of melamine
  • 文献类型:   Article
  • 作  者:   REN QX, SHEN XY, SUN YY, FAN RH, ZHANG J
  • 作者关键词:   branched polyethyleneimine, functionalized graphene, melamine immunosensor, competitive mode
  • 出版物名称:   FOOD CHEMISTRY
  • ISSN:   0308-8146 EI 1873-7072
  • 通讯作者地址:   Shenyang Med Coll
  • 被引频次:   4
  • DOI:   10.1016/j.foodchem.2019.125397
  • 出版年:   2020

▎ 摘  要

Branched polyethyleneimine functionalized reduced graphene oxide (BPEIGn) was prepared by a one step reaction, catalyzed by NaOH, using branched polyethyleneimine (BPEI) and graphene oxide (GO) without reductant hydrazine hydrate or sodium borohydride. The branched polyethylenimine acted as both a grafting agent and a reducing agent of GO. An competitive electrochemical immunosensor based on the Au/sodium mercaptopropanesulfonate/BPEIGn/gold nanoparticles/melamine (Au/MPS/BPEIGn/AuNPs/Mel) modified electrode was constructed for the determination of melamine. The double amplification of BPEIGn and AuNPs increased the sensitivity of the sensor. The melamine was detected by differential pulse voltammetry (DPV) in buffer solution (pH 7.4) containing K-3(Fe(CN)(6)]/K-4[Fe(CN)(6)]. Under optimized conditions, the proposed melamine immunosensor showed a linear relationship in the concentration range of 1x10(-6) to 1 mu M, with a detection limit of 2.66x10(-7) mu M.