▎ 摘　　要

Recent attention on chemotherapy against cancer is to explore the effective therapy through targeted delivery of anticancer agents to the tumor site by manipulating pharmacokinetic properties of nanocarriers. 5-Fluorouracil (5-FU) and curcumin (CUR) loaded chitosan/reduced graphene oxide (CS/rGO) nanocomposite has been prepared via simple chemical method. The polymer matrix-type chitosan/rGO nanocomposite, before and after encapsulation, has been analyzed by various characterizations. Entrapment and loading efficiencies were estimated. The results that demonstrated higher entrapment efficiency (> 90%) were achieved by CS/rGO nanocarrier. Various kinetic models were used to analyze the release model and to elucidate the release mechanism of the drug from CS/rGO nanocomposite. The synergistic cytotoxicity was observed on addition of 5-FU + CUR-loaded CS/rGO nanocomposite which shows the effectiveness of the system toward the inhibition of growth of HT-29 colon cancer cells. The better cytotoxicity with an IC50 of 23.8 mu g/mL was observed for dual-drug-loaded nanocomposite.