▎ 摘　　要

A composite monolithic column was prepared by redox initiation method for the on-line purification and enrichment of beta-sitosterol, in which graphene oxide (GO) was embedded. The obtained monolithic column was characterized by scanning electron microscopy (SEM) and nitrogen adsorption-desorption isotherm measurement, which indicated that the monolith possessed characteristics of porous structure and high permeability. Under the optimum conditions for extraction and determination, the calibration equation was y = 47.92 x -0.1391; the linear range was 0.008-1.0 mg mL(-1); the linear regression coefficient was 0.998; the limit of detection (LOD) is 2.4 mu g mL(-1); the limit of quantitation (LOQ) was 814 mu g mL(-1); precisions for intra-day and inter-day assays presented as relative standard deviations were less than 4.3% and 6.8%, respectively. Under the selective conditions, the enrichment factor of the method was 119. The recovery was in the range of 80.40-98.00%. Moreover, the adsorption amount of the monolith was compared with silica gel-C18 adsorbent and the monolith without graphene oxide being embedded. The polymerization monolithic column showed high selectivity and good permeability, and it was successfully used as on-line solid-phase extraction (SPE) column for determination of beta-sitosterol in edible oil.