• 文献标题:   A graphene oxide-based platform for the assay of RNA synthesis by RNA polymerase using a fluorescent peptide nucleic acid probe
  • 文献类型:   Article
  • 作  者:   PARK JS, BAEK A, PARK IS, JUN BH, KIM DE
  • 作者关键词:  
  • 出版物名称:   CHEMICAL COMMUNICATIONS
  • ISSN:   1359-7345 EI 1364-548X
  • 通讯作者地址:   Konkuk Univ
  • 被引频次:   19
  • DOI:   10.1039/c3cc45750h
  • 出版年:   2013

▎ 摘  要

We report a simple, direct fluorometric assay based on graphene oxide (GO) for RNA polymerase-mediated RNA synthesis. In principle, fluorescent peptide nucleic acid (PNA) probes were designed, and annealed with RNA products and the resultant RNA-PNA hybrids induced the recovery of fluorescence intensity of the PNA probes adsorbed onto the GO surface.