▎ 摘　　要

In this work, we report a methodology for the quantification of Homocysteine (HcySH) at neutral pH (pH-7.0) using Au nanoparticles incorporated reduced graphene oxide (AuNP/rGO/GCE) modified glassy carbon electrode. The modified electrode was characterized using SEM and XRD techniques. The electrode exhibited a typical behavior against the standard redox probe [Fe(CN)(6)](3-/4-) and resulted in 0.06 V peak to peak potential value. The modified electrode exhibited electrocatalytic activity towards electrochemical biosensing of HcySH, which is established using voltammetric studies. HcySH oxidation peak potential is observed at 0.12 V on AuNP/rGO/GCE which is 0.7 V cathodic than bare glassy carbon electrode (0.82V). The large peak potential shift observed is reasoned as the interaction of -SH group of HcySH with the gold nanoparticles and the electrocatalytic property of reduced graphene oxide that enhances the electrochemical detection at reduced over-potential. Further, successive addition of HcySH showed a linear increment in the sensitivity within the concentration range of 2-14 mM. From an amperometric protocol, the limit of detection is found as 6.9 mu M with a sensitivity of 14.8 nA/mu M. From a set of cyclic voltammetric measurements, it is observed that the electrode produces a linear signal on the concentration of HcySH in the presence of hydrogen peroxide. Thus it can be concluded that the matrix can detect HcySH even in the presence of hydrogen peroxide.