• 文献标题:   Porous graphene oxide nanostructure as an excellent scaffold for label-free electrochemical biosensor: Detection of cardiac troponin I
  • 文献类型:   Article
  • 作  者:   KAZEMI SH, GHODSI E, ABDOLLAHI S, NADRI S
  • 作者关键词:   immunosensor, porous graphene oxide, glassy carbon electrode, nanostructure, electrochemical impedance, cardiac troponini ctni
  • 出版物名称:   MATERIALS SCIENCE ENGINEERING CMATERIALS FOR BIOLOGICAL APPLICATIONS
  • ISSN:   0928-4931 EI 1873-0191
  • 通讯作者地址:   IASBS
  • 被引频次:   30
  • DOI:   10.1016/j.msec.2016.07.005
  • 出版年:   2016

▎ 摘  要

Herein, we report the fabrication of a novel label-free impedimenic biosensor employing porous graphene oxide (PrGO) nanostructures for the specific detection of cardiac troponin-I (cTnI) to establish the myocardial infarction (MI). This nano-immunosensor demonstrates an outstanding selectivity and high sensitivity towards the human-cTnI analyte. An excellent detection limit of 0.07 ng mL(-1) and dynamic linear range of 0.1-10 ng mL(-1) were calculated for anti-cTnI/PrGO/GG. Finally, this biosensor was employed to check the concentration of the MI biomarker in real clinical samples and the results are in good agreement with standard enzyme linked fluorescence assay (ELFA) method. (C) 2016 Elsevier B.V. All rights reserved.