• 文献标题:   Real-Time Monitoring of Insulin Using a Graphene Field-Effect Transistor Aptameric Nanosensor
  • 文献类型:   Article
  • 作  者:   HAO Z, ZHU YB, WANG XJ, ROTTI PG, DIMARCO C, TYLER SR, ZHAO XZ, ENGELHARDT JF, HONE J, LIN Q
  • 作者关键词:   affinity sensing aptamer, gquadruplex, graphene fieldeffect transistor gfet, insulin
  • 出版物名称:   ACS APPLIED MATERIALS INTERFACES
  • ISSN:   1944-8244
  • 通讯作者地址:   Columbia Univ
  • 被引频次:   18
  • DOI:   10.1021/acsami.7b07684
  • 出版年:   2017

▎ 摘  要

This paper presents an approach to the real-time, label-free, specific, and sensitive monitoring of insulin using a graphene aptameric nanosensor. The nanosensor is configured as a field-effect transistor, whose graphene-based conducting channel is functionalized with a guanine-rich IGA3 aptamer. The negatively charged aptamer folds into a compact and stable antiparallel or parallel G-quadruplex conformation upon binding with insulin, resulting in a change in the carrier density, and hence the electrical conductance, of the graphene. The change in the electrical conductance is then measured to enable the real-time monitoring of insulin levels. Testing has shown that the nanosensor offers an estimated limit of detection down to 35 pM and is functional in Krebs Ringer bicarbonate buffer, a standard pancreatic islet perfusion medium. These results demonstrate the potential utility of this approach in label-free monitoring of insulin and in timely prediction of accurate insulin dosage in clinical diagnostics.