▎ 摘　　要

Single-layered graphene, emerging as a true two-dimensional nanomaterial, has tremendous potential for electrochemical catalysis and biosensing as a novel electrode material. Considering the excellent properties of graphene, such as large surface-to-volume ratio, high conductivity and electron mobility at room temperature, low energy dynamics of electrons with atomic thickness, robust mechanical and flexibility, we give a general view of recent advances in electrochemical sensors based on graphene. We are highlighting here important applications of graphene and graphene nanocomposites, and the assay strategies in electrochemical sensors for DNA, proteins, neurotransmitters, phytohormones, pollutants, metal ions, gases, hydrogen peroxide, and in medical, enzymatic and immunosensors.