▎ 摘　　要

Detection of neurotransmitters is one of the most efficient methods for monitoring neurodegenerative diseases. Several electrochemical methods have been developed for neurotransmitter detection with conductive materials. In this study, gold-crumpled graphene (Au-crGr) composite modified electrodes were used to improve electrode conductivity and affinity with neurotransmitters. Au-crGr composites were synthesized at various component ratios and the structures were confirmed by electron microscopy. After the fabrication of Au-crGr composite modified electrodes, electrochemical methods and surface-enhanced Raman spectroscopy (SERS) were used to validate the spectroelectrochemical properties of various electrodes. The highest electrochemical and SERS signal intensity from a dopamine solution was obtained with Au-crGr composite modified electrodes. Moreover, secreted neurotransmitters from human neuroblastoma cells were successfully detected using the new material in a spectroelectrochemical method, and these results were confirmed by ultra-performance liquid chromatography and mass spectrometry. Our newly developed Au-crGr composite modified electrodes can be used as a catecholamine sensor and to study diagnostic methods for neurodegenerative diseases.