▎ 摘　　要

Infection from bacterial resistance to antibiotics has given rise to a grave threat to human health in the world. It is vital to developing highly efficient antibacterial materials that are safe and biocompatible with humans and without bacterial resistance. In this study, nanofiber membranes of graphene oxide/polyacrylonitrile (GO/PAN) with highly efficient antibacterial activity were fabricated via electrospinning technique. As the spindle-knot structure of membranes formed by the addition of GO sheets increased, the hydrophilicity and surface roughness increased. The antibacterial test indicated that antibacterial ratios of 3GO/PAN membranes against Escherichia coli and Staphylococcus aureus were 98.5% and 99.6%, respectively after contracting 24 h, with highly efficient antibacterial activity. Furthermore, the E. coli cell structures of adhered to the GO/PAN nanofiber surface changed significantly shrunk and deformed, and the number of S. aureus cell were obviously less contrast than the pure PAN. The main antibacterial mechanism was GO of spindle-knot in nanofiber membranes produced ROS destroyed the physiological activities of the bacteria lead to death. The fabricated GO/PAN nanofiber membrane of this study is promising to develop to a kind of novel antibacterial materials.