▎ 摘 要
Isolation and identification of glycoproteins are of great significance for the study of their biological functions , the discovery of more biomarkers and the development of new biological drugs. However, due to the complex composition of biological samples and the low abundance of glycoproteins , it is very challenging to isolate and identify glycoproteins. Therefore, it is very important to develop an efficient method for glycopeptides enrichment. Nitrogen carbamide (C2N) , a novel two-dimensional (2D) material, has high specific surface area, good hydrophilicity and abundant nitrogen, which provides the possibility of glycopeptides enrichment. In this study, a novel 2D C2N material was prepared for selective enrichment of glycopeptides. The pore size of the synthesized C2N was 2. 5 nm and the specific surface area was 633 m(2) /g. The enrichment method based on C2N showed many advantages such as high selectivity ( glycopeptides could be selectively enriched from the digests of bovine fetuin even mixed with the 200 mass folds of bovine serum albumin) , high adsorption capacity (150 mg/g) and high recovery (82. 2% +/- 7. 0%). The method was used for analysis of glycopeptide in 100 mu g of HeLa cell lysates using three technical replicates and a total of 339 , 353 and 327 glycosylation sites resulting from 328 , 320 and 316 glycopeptides were characterized, respectively. This study provided a new technique for glycoproteomic and a methodological basis for the discovery of potential tumor markers.