▎ 摘　　要

BACKGROUNDThis study describes, electrochemically prepared Pt-nanoparticles (PtNP) embedded poly(pyrrole-co-pyrrolepropylic acid) (PPy-PPa) copolymer film over reduced graphene oxide (RGO) sheets deposited on a silane modified indium-tin-oxide coated glass plate. The cardiac myoglobin protein antibody (Ab-Mb) was covalently immobilized on PtNP(PPy-PPa)-RGO nanocomposite through pendant carboxyl groups of the polymer chain using carbodiimide coupling reaction to construct a bioelectrode. The bioelectrode was characterized by high resolution transmission electron microscopy, energy dispersive spectra and electrochemical impedance spectroscopy (EIS). The sensing performance of the bioelectrode was investigated, as an impedimetric immunosensor, for the quantitative detection of the target human cardiac myoglobin antigen (Ag-cMb) in phosphate buffer silane (pH7.4). RESULTSThe EIS studies of the bioelectrode towards Ag-cMb showed a dominant charge transfer resistance (R-et) characteristic in the frequency region 50 to 100Hz. The EIS of the bioelectrode showed an effective immunoreaction with Ag-cMb with a significant increase in both R-et and the constant phase element parameter (n) from 0.61 to 0.85. The bioelectrode exhibited a linear dynamic range of detection from 10ngmL(-1) to 1 mu gmL(-1), sensitivity of 107.08 cm(2) per decade and a lowest detection limit of 4.0ngmL(-1) Ag-cMb. CONCLUSIONThe polymer nanocomposite with uniformly distributed PtNP in conjunction with conducting RGO support has been demonstrated to be a high performance immunosensor. This has been attributed to the synergistic combination of conducting RGO support and electroactive PtNP embedded conducting functional copolymer for efficient covalent biomolecular immobilization, responsible for high protein loading and fast interfacial electron exchange. (c) 2014 Society of Chemical Industry