▎ 摘　　要

A novel one-step electrochemical synthesis of the reduced graphene oxide and poly(m-aminobenzenesulfonic acid, ABSA) nanocomposite (PABSA-rGNO) via pulse potentiostatic method (PPM) for direct and freely switchable detection of target genes is presented. Unlike most electrochemical preparation of hybrids based on rGNO and polymer, electrochemical synthesis of PABSA (during the pulse electropolymerization period of PPM) and electrochemical reduction of rGNO (during the resting period of PPM), in this paper, were alternately performed. The total progress synchronously resulted in PABSA-rGNO nanocomposite. This nanocomposite was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), Fourier Transform infrared spectroscopy (FT-IR), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The PABSA-rGNO nanocomposite integrated graphene (a single-atom thick, two-dimensional sheet of sp(2) bonded conjugated carbon) with PABSA (owning rich-conjugated structures, functional groups, and excellent electrochemical activity), which could serve as an ideal electrode material for biosensing and electrochemical cell, etc. As an example, the immobilization of the specific probe DNA was successfully conducted via the noncovalent method due to the pi-pi* interaction between conjugated nanocomposite and DNA bases. The hybridization between the probe DNA and target DNA induced the product dsDNA to be released from conjugated nanocomposite, accompanied with the self-signal regeneration of nanocomposite ("signal-on"). The self-signal changes served as a powerful tool for direct and freely switchable detection of different target genes, and the synergistic effect of PABSA-rGNO nanocomposite effectively improved the sensitivity for the target DNA detection.