▎ 摘　　要

A novel and sensitive electrochemiluminescence (ECL) method for ethanol biosensor was developed by co-immobilizing the enzyme and ECL reagent Ru(bpy)(3)(2+) on the poly-(3,4-ethylene dioxythiophene) and polystyrene sulfonate functionalized graphene (PEDOT-PSS-G) nanocomposite film. Positively charged Ru(bpy)(3)(2+) could be immobilized effectively on the electrode surface with the negatively charged PSS and graphene, which provided a stable ECL platform for further modification with the enzyme. Moreover, the introduction of PEDOT and graphene can be acted as a conducting pathway to accelerate the electron transfer due to the high conductivity. Such biosensor combined enzymatic selectivity with the amplification of PEDOT-PSS-G performed well with a wide linear range, high sensitivity and good stability. The sensing platform was successfully applied to determine the amounts of alcohol in real samples.