▎ 摘　　要

Sensor technology demands to design a sensing system having higher sensitivity, sufficient selectivity, fast response time and efficient reproducibility with simple operating and recognition methodology. The chemical and biosensors with aforementioned features have been still challenging since several decades. Therefore we effectively designed graphene oxide nanocomposite based sensing system which exhibited remarkable electrocatalytic performance towards the recognition of key biomolecule, i.e., hydrogen peroxide (H2O2) in a single step which reflects strong interaction between graphene oxide based nanocomposite and H2O2. Graphene oxide based nanocomposite was prepared by low cost and low temperature facile method and characterized by different spectroscopic techniques. The electrochemical sensing of as prepared nanocomposite towards H2O2 was assessed using cyclic voltammetry (CV) and DC potential amperometry (DCPA at potential 0.570 V vs. Ag/AgCl reference electrode) in a phosphate buffer (pH = 7.0). The designed H2O2 biosensor displayed high sensitivity (-0.2844 +/- 0.0169 pA nM(-1) cm(-2)), low detection limit (5 nM) with wide linear dynamic range (0.01-0.05 mu M) and fast response time of less than 3 s. The performance of the sensor was optimized with various pH and different scan rates. Selectively, the biosensor recognized H2O2 in the presence of glucose, uric acid and ascorbic acid, as interfering agent. Further evaluation of designed biosensor was made on commercially available cosmetic product with state of the art percent recovery value. Precisely, the designed biosensor would be a promising candidate for the electrochemical recognition and quantification of H2O2. (C) 2016 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.