• 文献标题:   Femtosecond X-ray coherent diffraction of aligned amyloid fibrils on low background graphene
  • 文献类型:   Article
  • 作  者:   SEURING C, AYYER K, FILIPPAKI E, BARTHELMESS M, LONGCHAMP JN, RINGLER P, PARDINI T, WOJTAS DH, COLEMAN MA, DORNER K, FUGLERUD S, HAMMARIN G, HABENSTEIN B, LANGKILDE AE, LOQUET A, MEENTS A, RIEK R, STAHLBERG H, BOUTET S, HUNTER MS, KOGLIN J, LIANG MN, GINN HM, MILLANE RP, FRANK M, BARTY A, CHAPMAN HN
  • 作者关键词:  
  • 出版物名称:   NATURE COMMUNICATIONS
  • ISSN:   2041-1723
  • 通讯作者地址:   DESY
  • 被引频次:   11
  • DOI:   10.1038/s41467-018-04116-9
  • 出版年:   2018

▎ 摘  要

Here we present a new approach to diffraction imaging of amyloid fibrils, combining a freestanding graphene support and single nanofocused X-ray pulses of femtosecond duration from an X-ray free-electron laser. Due to the very low background scattering from the graphene support and mutual alignment of filaments, diffraction from tobacco mosaic virus (TMV) filaments and amyloid protofibrils is obtained to 2.7 A and 2.4 A resolution in single diffraction patterns, respectively. Some TMV diffraction patterns exhibit asymmetry that indicates the presence of a limited number of axial rotations in the XFEL focus. Signal-to-noise levels from individual diffraction patterns are enhanced using computational alignment and merging, giving patterns that are superior to those obtainable from synchrotron radiation sources. We anticipate that our approach will be a starting point for further investigations into unsolved structures of filaments and other weakly scattering objects.