▎ 摘　　要

An optosensor using nanocomposite probes was fabricated for the detection of trace cefazolin. The nanoprobes utilized the high affinity of titanium dioxide, the good optical properties of graphene quantum dots and the good selectivity of molecularly imprinted polymer. The integration of these materials produced a rapid, highly sensitive optosensor with excellent selectivity for cefazolin detection. The fluorescence intensity of the nanocomposite probes was quenched when cefazolin re-bound with the imprinted recognition cavities of the nanoprobes. The fabricated nanoprobe exhibited a good linearity for cefazolin from 0.10 to 10.0 mu g L-1 with a limit of detection of 0.10 mu g L-1. The imprinting factor of the nanoprobe was 10.6 and selectivity for cefazolin was not affected by the analogue structures of cephalexin, cefatriaxone, cephradine, cefaperazone and ceftazidime. This nano-optosensor probe successfully detected cefazolin in milk and recoveries were between 85.0 and 97.4 % with RSDs less than 5.0 %. The results of analysis with nano-optosensor were in good agreement with HPLC analysis. The fabrication strategy of the nanocomposite probe can be modified for the measurement of other toxic compounds. (C) 2020 Elsevier B.V. All rights reserved.