▎ 摘　　要

In this paper three-dimensional graphene (3DGR) was synthesized by hydrothermal method and further used as electrode modifier on the surface of carbon ionic liquid electrode (CILE). Then an electrochemical biosensor was prepared by further immobilizing myoglobin (Mb) and chitosan (CTS) on the electrode surface in sequence. Spectral results showed that Mb immobilized with 3DGR maintained its original structure. Cyclic voltammogram of CTS/Mb/3DGR/CILE in pH 4.0 phosphate buffer solution gave a pair of quasi-reversible redox peak, indicating that direct electrochemistry of Mb had been achieved on the electrode, which was attributed to the specific macrostructure of 3DGR with high conductivity and large interconnected network. By using CTS/Mb/3DGR/CILE as the working electrode, a third-generation electrochemical biosensor was constructed for the detection of trichloroacetic acid with the linear range from 16.0 to 80.0 mmol/L and the detection limits calculated as 5.33 mmol/L. Experimental results extended the application of 3DGR in the electrode modification with advantages such as high sensitivity, wide detection range, good stability and reproducibility.