• 文献标题:   Integration of molecular and enzymatic catalysts on graphene for biomimetic generation of antithrombotic species
  • 文献类型:   Article
  • 作  者:   XUE T, PENG B, XUE M, ZHONG X, CHIU CY, YANG S, QU YQ, RUAN LY, JIANG S, DUBIN S, KANER RB, ZINK JI, MEYERHOFF ME, DUAN XF, HUANG Y
  • 作者关键词:  
  • 出版物名称:   NATURE COMMUNICATIONS
  • ISSN:   2041-1723
  • 通讯作者地址:   Univ Michigan
  • 被引频次:   62
  • DOI:   10.1038/ncomms4200
  • 出版年:   2014

▎ 摘  要

The integration of multiple synergistic catalytic systems can enable the creation of biocompatible enzymatic mimics for cascading reactions under physiologically relevant conditions. Here we report the design of a graphene-haemin-glucose oxidase conjugate as a tandem catalyst, in which graphene functions as a unique support to integrate molecular catalyst haemin and enzymatic catalyst glucose oxidase for biomimetic generation of antithrombotic species. Monomeric haemin can be conjugated with graphene through pi-pi interactions to function as an effective catalyst for the oxidation of endogenous L-arginine by hydrogen peroxide. Furthermore, glucose oxidase can be covalently linked onto graphene for local generation of hydrogen peroxide through the oxidation of blood glucose. Thus, the integrated graphene-haemin-glucose oxidase catalysts can readily enable the continuous generation of nitroxyl, an antithrombotic species, from physiologically abundant glucose and L-arginine. Finally, we demonstrate that the conjugates can be embedded within polyurethane to create a long-lasting antithrombotic coating for blood-contacting biomedical devices.