▎ 摘　　要

The purpose of this research was to develop a poly(cyanocobalamin) modified composite of Ag nanoparticles graphene oxide (poly(cyc)/Ag-GO) for electrochemical monitoring of cisplatin, a chemotherapy medication used to treat laryngeal cancer. The Ag-GO composite was electrodeposied on a glassy carbon electrode (GCE), and then poly(cyc) was electropolymerized on Ag-GO/GCE. The XRD and FE-SEM structural characterization of Ag-GO nanocomposite revealed that the Ag nanoparticles were discretely distributed on the surface of the GO nanosheets, resulting in a high porous structure with discrete electroactive sites for cyanocobalamin's strong bond and improved electron transfer and signal in electrochemical reactions. Using CV and DPV, electrochemical analyses revealed a stable, sensitive, and selective response of poly(cyc)/Ag-GO/GCE to cisplatin determination. The proposed sensor's detection limit, sensitivity, and linear range were determined to be 0.2 mu M, 0.01316 mu A/mu M, and 40-1200 mu M, respectively. The sensor's capability was tested using prepared platinol injection and human serum as real samples, and the results revealed acceptable recovery (95.25% to 98.50%) and RSD (3.08% to 4.76%), implying that the proposed cisplatin sensor is accurate enough for clinical sample analysis.