▎ 摘　　要

The cell-chip coupling is in general regulated by the interplay between cells and the material surface at the interface. Electroactive planar materials have shown limited crosstalk with cells, whereas pseudo 3D patterned materials promote a more intimate contact with the biological system. Here, unprecedented physical properties of a carbon-based material, i.e., graphene, to engineer out-of-plane morphologies are exploited: 1) 3D single- to few-layer fuzzy graphene morphology (3DFG), 2) 3DFG on a collapsed Si nanowire mesh template, and 3) 3DFG on a noncollapsed Si nanowire mesh template. These materials are synthesized and interfaced with cardiomyocyte-like cells focusing on the characterization of the cytoskeletal arrangement as well as membrane wrapping processes yet regulated by endocytic proteins. Finally, some major conditions to promote tight coupling to the device and eventually spontaneous intracellular penetration are found.