▎ 摘　　要

Three-dimensional layered silver nanoflowers/graphene (AgNFs/Graphene) nanohybrids were firstly proposed and fabricated for the sensitive flexible biosensors. Flexible bio-friendly graphene films, which are beneficial for the Ag adatom absorption, can be used not only as the growth platform to form the AgNFs nanostructures, but also as the nano-spacer to form the layered AgNFs/Graphene nanohybrids. The growth mechanism and the horizon-vertical (H-V) coupling effects of the porous AgNFs/Graphene layers were studied by using VASP and COMSOL simulation. Electromagnetic intensity can be easily enhanced by the horizontal coupling on the AgNFs nanostructures surfaces and the vertical coupling on the layered AgNFs/Graphene nanohybrids. Rhodamine 6 G (R6 G), CV, lysozyme, and DNA molecules can be easily detected by our proposed flexible biosensors.