▎ 摘　　要

Polyvinyl alcohol-ionic liquids (PVA-ILs) composite sol, graphenes( GS) and nano-Au were used to immobilize horseradish peroxidase(HRP). A novel hydrogen peroxide biosensor(HRP/PVA-ILs/HRP/nano Au-GS/GC) was obtained by immobilizing HRP with composite membrane of PVA-ILs on a glassy carbon (CC) electrode, which was modified with graphenes and nano Au. The experimental condition was optimized and the electrochemistry behavior of the HRP/PVA-ILs/HRP/nano Au-GS/GC biosensor in 0. 1 mol/L HAc-NaAc+0.1 mol/L KCl( pH =6. 5) including 11202 was discussed. The response of the sensor to 11202 is linear in the range of 9. 55x10(-6)-6.01x10(-3) mol/L with a correlation coefficient of 0.9990. The detection limit is 3.3 x10(-7) mol/L. The experiments show that the biosensor exhibits high sensitivity, good reproducibility and stability and longer service life.