• 文献标题:   Polyaniline-graphene based alpha-amylase biosensor with a linear dynamic range in excess of 6 orders of magnitude
  • 文献类型:   Article
  • 作  者:   TEIXEIRA SR, LLOYD C, YAO S, GAZZE AS, WHITAKER IS, FRANCIS L, CONLAN RS, AZZOPARDI E
  • 作者关键词:   immunosensor, screenprinted electrode, graphene, antibody, polyaniline, alphaamylase
  • 出版物名称:   BIOSENSORS BIOELECTRONICS
  • ISSN:   0956-5663 EI 1873-4235
  • 通讯作者地址:   Swansea Univ
  • 被引频次:   16
  • DOI:   10.1016/j.bios.2016.05.034
  • 出版年:   2016

▎ 摘  要

alpha-amylase is an established marker for diagnosis of pancreatic and salivary disease, and recent research has seen a substantial expansion of its use in therapeutic and diagnostic applications for infection, cancer and wound healing. The lack of bedside monitoring devices for a-amylase detection has hitherto restricted the clinical progress of such applications. We have developed a highly sensitive alpha-amylase immunosensor platform, produced via in situ electropolymerization of aniline onto a screen-printed graphene support (SPE). Covalently binding an a amylase specific antibody to a polyaniline (PANT) layer and controlling device assembly using electrochemical impedance spectroscopy (EIS), we have achieved a highly linear response against alpha-amylase concentration. Each stage of the assembly was characterized using a suite of high-resolution topographical, chemical and mechanical techniques. Quantitative, highly sensitive detection was demonstrated using an artificially spiked human blood plasma samples. The device has a remarkably wide limit of quantification (0.025-100010) compared to alpha-amylase assays in current clinical use. With potential for simple scale up to volume manufacturing though standard semiconductor production techniques and subsequently clinical application, this biosensor will enable clinical benefit through early disease detection, and better informed administration of correct therapeutic dose of drugs used to treat alpha-amylase related diseases. (C) 2016 Elsevier B.V. All rights reserved.