• 文献标题:   Colorimetric and electrochemical assay for dual-mode detection of di-2-ethylhexyl phthalate based on hemin-graphene nanocomposites
  • 文献类型:   Article
  • 作  者:   ZHANG YJ, HAN YJ, LIU ZG, FAN LF, GUO YJ
  • 作者关键词:   hemingraphene, aptamer, colorimetric detection, electrochemical detection, di2ethylhexyl phthalate detection
  • 出版物名称:   MICROCHEMICAL JOURNAL
  • ISSN:   0026-265X EI 1095-9149
  • 通讯作者地址:  
  • 被引频次:   0
  • DOI:   10.1016/j.microc.2023.108788 EA APR 2023
  • 出版年:   2023

▎ 摘  要

Di-2-ethylhexyl phthalate (DEHP) is one of the phthalic acid esters (PAEs) that can impact human health and increase the risk of cancer. In this study, we report a dual-mode method for detecting DEHP using hemin-graphene (H-Gr) hybrids and DEHP aptamers. The H-Gr nanocomposites possess inherent peroxidase-like ac-tivity, which can catalyze the chromogenic substrate TMB to produce a blue oxidized product (oxTMB). Addi-tionally, H-Gr can be used as an in situ probe for electrochemical detection. And DEHP aptamer is added as the biological recognition element. Due to its specific recognition properties, the DEHP-aptamer complexes formed inhibit the enzymatic activity and reduce the electrochemical signal of H-Gr. As a result, colorimetric and electrochemical methods were developed for detecting DEHP with a linear relationship ranging from 2.00 x 10(-10) -1.00 x 10-7 g center dot L-1 and 1.00 x 10(-10) -1.00 x 10(-7) g center dot L-1, respectively. In summary, our study presents a sensitive, rapid, and simple dual-mode strategy for detecting DEHP and has the potential for practical use in detecting DEHP in water samples.