• 文献标题:   Fluorometric determination of cardiac myoglobin based on energy transfer from a pyrene-labeled aptamer to graphene oxide
  • 文献类型:   Article
  • 作  者:   LIU DK, ZENG YB, ZHOU GB, LU X, MIAO DW, YANG YW, ZHAI YY, ZHANG J, ZHANG ZL, WANG HL, LI L
  • 作者关键词:   chemical modification, pmama probe, fret, fluorophore, fluorescent recovery
  • 出版物名称:   MICROCHIMICA ACTA
  • ISSN:   0026-3672 EI 1436-5073
  • 通讯作者地址:   Jiaxing Univ
  • 被引频次:   2
  • DOI:   10.1007/s00604-019-3385-x
  • 出版年:   2019

▎ 摘  要

The authors describe a fluorometric assay for cardiac myoglobin (Mb), a marker for myocardial infarction. An Mb-binding aptamer was labeled with pyrene and adsorbed on the surface of graphene oxide (GO) via noncovalent and reversible binding forces. This causes the fluorescence of pyrene (best measured at excitation/emission wavelengths of 275/376nm) to be quenched. However, fluorescence is restored on addition of pyrene due to the strong affinity between Mb and aptamer which causes its separation from GO. Fluorescence increases linearly in the 5.6-450 pM Mb concentration range, and the lower detection limit is 3.9 pM (S/N=3). The assay was applied to the determination of cardiac Mb in spiked serum, and satisfactory results were obtained.