▎ 摘　　要

In this work, the modified carbon paste electrode (CPE) with an imidazole derivative 2-(2,3 dihydroxy phenyl) 4-methyl benzimidazole (DHPMB) and reduced graphene oxide (RGO) was used as an electrochemical sensor for electrocatalytic oxidation of N-acetyl-L-cysteine (NAC). The electrocatalytic oxidation of N-acetyl-L-cysteine on the modified electrode surface was then investigated, indicating a reduction in oxidative over voltage and an intensive increase in the current of analyte. The scan rate potential, the percentages of DHPMB and RGO, and the pH solution were optimized. Under the optimum conditions, some parameters such as the electron transfer coefficient (a) between electrode and modifier, and the electron transfer rate constant) k(s)) in a 0.1 M phosphate buffer solution (pH=7.0) were obtained by cyclic voltammetry method. The diffusion coefficient of species (D) 3.96 x 10(-5) cm(2) s(-1) was calculated by chronoamperometeric technique and the Tafel plot was used to calculate a (0.46) for N-acetyl-L-cysteine. Also, by using differential pulse voltammetric (DPV) technique, two linear dynamic ranges of 2-18 mu M and 18-1000 mu M with the detection limit of 61.0 nM for N-acetyl-L-cysteine (NAC) were achieved. In the co-existence system of N-acetyl-L-cysteine (NAC), uric acid (UA) and dopamine (DA), the linear response ranges for NAC, UA, and DA are 6.0-400.0 mu M, 5.0-50.0 mu M and 2.0-20.0 mM, respectively and the detection limits based on (C= 3s(b)/m) are 0.067 mu M, 0.246 mu M and 0.136 mu M, respectively. The obtained results indicated that DHPMB/RGO/CPE is applicable to separate NAC, uric acid (UA) and dopamine (DA) oxidative peaks, simultaneously. For analytic performance, the mentioned modified electrode was used for determination of NAC in the drug samples with acceptable results, and the simultaneous determination of NAC, UA and DA oxidative peaks was investigated in the serum solutions, too.