▎ 摘　　要

The reliable detection of glucose is desirable for various applications. Herein, a nonenzymatic glucose electrochemical sensor based on copper (Cu)-nickel (Ni) bimetallic nanocatalyst anchored on reduced graphene oxide (rGO) modified glassy carbon electrode (GCE) was fabricated. The controllable and in-situ formation of rGO/Cu-Ni nanocomposites onto GCE was achieved via a one-step electrodeposition strategy. The chemical constituents, crystallization degrees, surface morphologies, and electrocatalytic features of nanocomposites were characterized with different techniques. The electrochemical sensor exhibited superior synergistic effects towards electrocatalytic oxidation of glucose in alkaline solution, and was applied to determine glucose in human serum sample with satisfactory results. The universal methodologies for hybrid nanocomposites preparation and electrochemical platforms fabrication can be exploited in other food or pharmaceutical analysis.