▎ 摘　　要

Facile and green fabrication of reduced graphene oxide on loofah sponge (rGOLS) carrier was applied for cell immobilization of e-Poly -L-lysine (epsilon-PL) production. Due to surface properties including large specific surface area, high polarity, and low interaction energy, rGOLS-1 was employed as the optimum rGOLS to enhance immobilization of Streptomyces albulus. Compared with raw LS, batch experiments showed rGOLS-1 facilitated superior cell vitality for epsilon-PL production due to the presence of reduced graphene oxide. In the sequential fed batch cultivation of Streptomyces albulus using rGOLS-1 with an aerobic plant fibrous-bed bioreactor (rGOLS-1AFPB), the maximum epsilon-PL concentration and productivity reached to 39.2 +/- 0.63 g/L and 0.48 g/L/h. The cells immobilized in rGOLS-1 with high vitality and epsilon-PL production efficiency were reused six times over a period of 624 h. This research afforded an effective approach to enhance the fermentation performance of immobilized cells with the design of an advanced immobilization carrier.