▎ 摘　　要

In this work a novel simple, inexpensive and highly sensitive approach for the antibody assisted detection of the human epidermal growth factor receptor 2 (HER2) is presented. Three sensors using carboxylic group containing gold functionalized graphene quantum dots (SNGQDs@AuNPs), CoP-BNF (cobalt porphyrin binuclear framework) and CoP-BNF/SNGQDs@AuNPs (the conjugate) nanocomposites on a GCE were designed. The sensors were fabricated by covalent immobilization of the antibody to the GCE via an amide bond. The modified GCE was characterized using cyclic voltammetry and impedance spectroscopy. The performance of the sensors was assessed using electrochemical impedance spectroscopy. The selective recognition of the antibody towards HER2 on the electrode led to an increase of the electron transfer resistance (R-ct). The different modified electrodes had low detection limit values: 0.0327 ng/mL, 0.0454 ng/mL and 0.1072 ng/mL towards the detection of HER2. The sensors showed good stability and reproducibility. The sensors showed promising potential in clinical applicability, in the detection of HER2 protein in human serum.